ZFIN Image: Dupret et al., 2016, Fig. 8

Image

Figure Caption

Fig. 8

Phenotype of 12 month-old pcgf1^-/- mutants.

(A) Pictures of 12 month-old pcgf1^+/+ and pcgf1^-/- fish. Note that both male and female pcgf1^-/- fish exhibit mild spinal curvature. (B) Measurements of the spinal curvature score in 12 month-old fish from the pcgf1^+/+ and pcgf1^-/- populations. Statistical significance was assessed by Student t-test analysis and significance expressed as the value of p. (C) Senescence-associated β-galactosidase (SA-β-Gal) activity in 12 month-old zebrafish. Whole body staining of SA-β-Gal activity in pcgf1^+/+ and pcgf1^-/- fish harboring a spinal curvature are compared. (D) Quantification of SA-β-Gal activity in pcgf1^+/+ and pcgf1^-/- fish. Blue pixels obtained by whole body staining of SA-β-Gal activity were quantified in the trunk area between the operculum and the dorsal and anal fins by Adobe Photoshop and plotted using Prism (GraphPad). Error bars indicate ± SD.

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One