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Fig. 7
Cell proliferation measured by whole-mount immunohistochemistry using an anti-phosphohistone H3 antibody.
(A) Antibody staining against phosphorylated histone H3 (anti-H3S10p) in pcgf1+/+ and pcgf1-/- 24 hpf embryos. The caudal fin fold region of representative embryos is shown. (B) Quantification of H3S10p spots in the caudal fin fold expressed per unit of surface since pcgf1+/+ and pcgf1-/- embryos have different sizes at this developmental stage. Error bars indicate ± SD. Statistical significance was assessed by Student t-test analysis and significance expressed as the value of p and * indicates p < 0.01.