PUBLICATION
The Polycomb Group Protein Pcgf1 Is Dispensable in Zebrafish but Involved in Early Growth and Aging
- Authors
- Dupret, B., Völkel, P., Le Bourhis, X., Angrand, P.O.
- ID
- ZDB-PUB-160722-2
- Date
- 2016
- Source
- PLoS One 11: e0158700 (Journal)
- Registered Authors
- Angrand, Pierre-Olivier, Völkel, Pamela
- Keywords
- Zebrafish, Embryos, Histones, TALENs, Larvae, 3' UTR, Fishes, In situ hybridization
- MeSH Terms
-
- Cartilage/embryology
- Cartilage/metabolism
- Mutation/genetics
- Sequence Homology, Amino Acid
- Cell Proliferation
- Transcription Activator-Like Effector Nucleases
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- Repressor Proteins/genetics*
- Repressor Proteins/metabolism
- Base Sequence
- Histones/metabolism
- Regeneration
- Immunohistochemistry
- Skull/blood supply
- Skull/embryology
- Skull/metabolism
- Gene Expression Regulation, Developmental
- Zebrafish/embryology*
- Zebrafish/genetics*
- Aging/genetics*
- Animal Fins/physiology
- Phenotype
- Embryonic Development/genetics*
- Polycomb Repressive Complex 1/genetics*
- Polycomb Repressive Complex 1/metabolism
- Phosphoproteins/metabolism
- Animals
- Bone and Bones/embryology
- Bone and Bones/metabolism
- PubMed
- 27442247 Full text @ PLoS One
Citation
Dupret, B., Völkel, P., Le Bourhis, X., Angrand, P.O. (2016) The Polycomb Group Protein Pcgf1 Is Dispensable in Zebrafish but Involved in Early Growth and Aging. PLoS One. 11:e0158700.
Abstract
Polycomb Repressive Complex (PRC) 1 regulates the control of gene expression programs via chromatin structure reorganization. Through mutual exclusion, different PCGF members generate a variety of PRC1 complexes with potentially distinct cellular functions. In this context, the molecular function of each of the PCGF family members remains elusive. The study of PCGF family member expression in zebrafish development and during caudal fin regeneration reveals that the zebrafish pcgf genes are subjected to different regulations and that all PRC1 complexes in terms of Pcgf subunit composition are not always present in the same tissues. To unveil the function of Pcgf1 in zebrafish, a mutant line was generated using the TALEN technology. Mutant pcgf1-/- fish are viable and fertile, but the growth rate at early developmental stages is reduced in absence of pcgf1 gene function and a significant number of pcgf1-/- fish show signs of premature aging. This first vertebrate model lacking Pcgf1 function shows that this Polycomb Group protein is involved in cell proliferation during early embryogenesis and establishes a link between epigenetics and aging.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping