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Fig. 7
Atp1b1a is required for epidermal integrity and polarity.
(a-h). Whole mount IFs of Cdh1 (red) and Lgl2 (green) in 54 hpf embryos raised in hypotonic (a,b,e,f) and isotonic (c,d,g,h) conditions. In mutants, localization of Cdh1 (b, d, f, and h; compare to wt a, c, e, g) and Lgl2 (b′, d′, f′, and h′; compare to wt a′, c′, e′, g′) is compromised in both peridermal cells (a-d) and basal cells (e-h). Images show regions of the trunk epidermis not yet affected by aggregate formation. (i- l). Whole mount IFs of cytokeratin (red) in 84 hpf embryos show a reduction of basally localized cytokeratin in mutants raised in hypotonic (j) and isotonic (l) conditions. Images show regions of the trunk epidermis above the yolk sac extension. (m-p). Live images of the tail fins of 54 hpf embryos either with full MO knockdown of atp1b1a (m) or with partial MO knockdown of lgl2 (n), of atp1b1a (o), or of both (p). (q) Quantification of the phenotypes of 54 hpf embryos in synergistic enhancement studies; n = 31-88. Similar results were obtained in two additional independent experiments.