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Fig. 1 Generation of pax7a and pax7b mutants using TALENs. Schematic overview of TALEN-induced deletions resulting in frameshift mutations in genes (A) pax7a and (B) pax7b on chromosome 11. Black arrows represent the left and right TALEN sequences, and the gray box represents the spacer sequence indicating the FokI cut site. Purple indicates the insertion of mismatched nucleotides, and red indicates mismatched amino acids. (C) Lateral view of 24-hpf wt embryo and pax7a/pax7b double-mutant trunk immunofluorescently analyzed using a Pax7 antibody (red). DAPI is used to visualize nuclei.
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