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Fig. 7
TLR2 blockade ameliorated the kidney injury induced by LipL32.
(A) Pronephric kidney injuries were induced in the lipl32 mRNA-injected wt1b:GFP larvae at 48 hpf. Representative images of normal, mild and severe deformities in pronephros are shown. Morphological deformities were assessed according to the criteria described in Methods. Photos were collected under fluorescence microscopy (dorsal view, anterior to the top). Scale bar, 50 µm. (B) Morpholino (MO) knockdown of tlr2 but not tlr4a and tlr4b significantly attenuated the LipL32-induced kidney injuries. The diagram indicates the frequencies of normal, mild and severe deformities in the wt1b:GFP larvae microinjected with different TLR MOs (n = 32 to 83 from three experiments, *P < 0.05, **P < 0.01, ***P < 0.001 versus lipl32 mRNA, n.s., not significant).