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Fig. 5
LipL32 promoted pronephric inflammation through the TL2R pathway in zebrafish.
Morpholino (MO) knockdown of tlr2 but not tlr4a and tlr4b attenuated the expression of l-plastin induced by microinjection of myc-tagged lipl32 mRNA. (A) In situ hybridization for l-plastin in zebrafish larvae at 48 hpf, with MO-knockdown of tlr2, myd88 and tlr4a/4b in response to lipl32-myc mRNA injection. Wild-type (WT) larvae and myc mRNA-injected control larvae are shown. All panels are lateral view (head toward the left). The insets are enlarged views of the corresponding boxed regions containing the pronephric ducts. (B) Comparative frequencies of strong and weak staining of l-plastin with different MOs (n = 43 to 171 from two experiments, ***P < 0.0001 versus lipl32 mRNA). (C) Quantitative real-time RT-PCR analyses show that tlr2 MO inhibited the expression of l-plastin induced by LipL32. Results shown are the mean ± SEM from three independent experiments carried out in duplicate (*P < 0.05 versus controls, #P < 0.05 versus LipL32). Scale bar, 200 µm.