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Fig. 1
Inhibition of Yap/Taz transcriptional activity in endothelial cells downregulates intramembranous ossification. (a) 293T cells were transfected with pFR-Luc and pcDNA3.1-Gal4-hTead2ΔN for measuring the transcriptional activities of zYap or zTaz. Cells were also introduced with p3xflag-cmv-14-zYap, -zTaz, and pEGFP-hTead2ΔN, as indicated. 24 hours after transfection, cells were harvested and measured the transcriptional activities of zYap or zTaz by luciferase activity. Control cells were introduced pFR-Luc and pcDNA3.1-Gal4-hTead2ΔN with or without pEGFP-hTead2ΔN. Average and SD were calculated from 3 experiments. (b,c) Control Tg(fli1: gal4-vp16) embryos and Tg(fli1: gal4-vp16): (UAS: GFP-htead2ΔN) were stained with alizarin red s, and the volume of the opercle (op), cleithrum (cl), and the diameter of the otolith (ot) at indicated hours post fertilization (hpf) were measured. The Branchiostegal ray (bsr) is also indicated. Average and SD were calculated from more than 13 embryos in each sample. Bar: 50 µm. (d,e) Similar experiments to (b,c) were performed using Tg(fli1: gal4-vp16): (UAS: GFP-hyapN). Average and SD were calculated from more than 11 embryos in each sample. Bar: 50 µm.