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Fig. S4
Hoxb8a knockdown leads to reduced proliferation in the primordium. BrdU incorporation (red) in cldnb:lyngfp (green) embryos in (A) control and (B) Hoxb8a morphants at 24 hfp. The leading zone is defined as the region containing primordium cells caudal to the first forming proneuromast L1. Histograms of the total cell number in (C) L1 and (D) the leading zone of control (n = 11) and Hoxb8a (n = 10) morphant primordia at 24 hpf. Histograms showing the proportion of BrdU postitive cells in (E) L1 and (F) the leading zone of control (n = 11) and Hoxb8a (n = 10) morphant primordia at 24 hpf. **P < 0.005; ***P < 0.001.