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Fig. 4
Whole-mount immunofluorescence staining of the 4 dpf zebrafish larvae (a), 22 dpf juvenile zebrafish (b) using antibody against galanin and adrenergic marker-tyrosine hydroxylase (TH). Arrow points to galaninergic neurons in autonomic ganglia. Arrowheads show the primary islet of the endocrine pancreas. Galanin-IR cells and fibers did not express immunoreactivity to TH. However, very intensely stained TH-IR neurons were found in the celiac ganglion (a) and in the brain (b). CeG celiac ganglion, E eye, I intestine, Me mesencephalon, Rho rhombencephalon. Scale bars 100 µm
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Full text @ Histochem. Cell Biol.