PUBLICATION

Galanin regulates blood glucose level in the zebrafish: a morphological and functional study

Authors
Podlasz, P., Jakimiuk, A., Chmielewska-Krzesinska, M., Kasica, N., Nowik, N., Kaleczyc, J.
ID
ZDB-PUB-151027-16
Date
2016
Source
Histochemistry and cell biology   145(1): 105-17 (Journal)
Registered Authors
Fedorowska - Nowik, Natalia, Jakimiuk, Anna, Kasica, Natalia, Podlasz, Piotr
Keywords
Blood glucose, Galanin, Insulin, Parasympathetic ganglia, Somatostatin, Zebrafish
MeSH Terms
  • Animals
  • Blood Glucose/analysis*
  • Blood Glucose/drug effects
  • Female
  • Galanin/analogs & derivatives
  • Galanin/pharmacology*
  • Ganglia, Parasympathetic/metabolism
  • Islets of Langerhans/metabolism*
  • Lipopeptides/pharmacology
  • Male
  • Receptors, Galanin/agonists*
  • Zebrafish
PubMed
26496922 Full text @ Histochem. Cell Biol.
Abstract
The present study has demonstrated the galaninergic innervation of the endocrine pancreas including sources of the galaninergic nerve fibers, and the influence of galanin receptor agonists on blood glucose level in the zebrafish. For the first time, a very abundant galaninergic innervation of the endocrine pancreas during development is shown, from the second day post-fertilization to adulthood. The fibers originated from ganglia consisting of galanin-IR, non-adrenergic (non-sensory) neurons located rostrally to the pancreatic tissue. The ganglia were found on the dorsal side of the initial part of the anterior intestinal segment, close to the intestinal branch of the vagus nerve. The galanin-IR neurons did not show immunoreactivity for applied antibodies against tyrosine hydroxylase, choline acetyltransferase, and vesicular acetylcholine transporter. Intraperitoneal injections of galanin analog NAX 5055 resulted in a statistically significant increase in the blood glucose level. Injections of another galanin receptor agonist, galnon, also caused a rise in blood glucose level; however, it was not statistically significant. The present findings suggest that, like in mammals, in the zebrafish galanin is involved in the regulation of blood glucose level. However, further studies are needed to elucidate the exact mechanism of the galanin action.
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