Image
|
Figure Caption
Fig. 3
Reverse-transcription quantitative PCR (RT-qPCR) analysis demonstrates that disc1 expression is altered in sonic hedgehog pathway mutants. (A) Graphical representation demonstrating that expression of disc1 was below the threshold for detection in smob641 mutant embryos at 50hpf. (B) Expression of disc1 was 0.65-fold lower in ptch1;ptch2 double mutants than siblings (P=0.0027). Both ptch1;ptch2 mutant (C; 1.8-fold, P=0.035) and sibling (D; 2.4-fold, P<0.0001) pools show increased disc1 expression relative to smob641 sibling embryos at 50hpf. (n=4; *P<0.05, **P<0.005, ****P<0.0001; error bars show s.e.m.).
Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ Biol. Open