IMAGE

Fig. S6

ID

ZDB-IMAGE-160518-12

Genes

strip2

Antibodies

Publication

Eden et al., 2016 - Myoscape controls cardiac calcium cycling and contractility via regulation of L-type calcium channel surface expression

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Figures for Eden et al., 2016

Image

Figure Caption

Fig. S6

(a left panel) RT -PCR analysis in zebrafish hearts from Myoscape knockdown morphants compared to controls showed a successful splice site mutation as indicated by a clear double band. (a right panel) Western blot analysis of zebrafish hearts from Myoscape knockdown morphants compared to controls, revealing a significant (>60%) reduction of Myoscape protein content after morpholino injection. (b+d) Histological (HE staining, scale bar 100µm), immunohistochemical (scale bar 100µm) (c) and ultrastructural evaluation of zebrafish heart (e) sections and skeletal muscle tissue (f) showing no gross architectural deficits after Myoscape ablation, indicating no obvious role of Myoscape in development or cellular sub-architecture of these tissues in zebrafish (scale bar EM 1 µm).

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.