Fig. S5

Fig. S5

Pharmacological inhibition of the RA pathway expands the CMZ ventrally along the choroid fissure. (A-B) Lateral views of eyes from wild-type gdf6a^u768 siblings treated with vehicle (DMSO, A) or the RAR inverse agonist BMS493 (15 µM, B) and assayed for expression of a marker of the putative stem compartment of the CMZ (col15a1b). Boxed regions in A and B were imaged with a 40x lens and are shown A′ and B′, respectively. Arrow indicates choroid fissure. (C) Total eye area and col15a1b-positive area were measured for lateral views of either vehicle or BMS493-treated homozygous mutant eyes (n≥11 embryos), and percentage of eye area occupied by col15a1b-expressing cells was calculated for each eye. Mean percent col15a1b-positive areas were then graphed with standard error bars (95% confidence limits; **Student’s t-test, p=0.0054).