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Fig. S7
Purity of FACS-purified rods. Representative microscopic images of a purified rod fraction from ES1-TG. Rod cell bodies were identified by signals for both EGFP (green) and nuclei (red, stained with DAPI). Approximately 97% of nuclei-containing dots were EGFP-positive. Few dots showed signal only for either EGFP (arrow heads) or nuclei (arrows), which were derived from rod outer segment or other cells, respectively. Possible contamination of cones was also investigated by immunostaining with antibodies against cone arrestins. Population of cone arrestins-immunopositive cells was only 0.28%.
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