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Fig. S6
lnx2a-MO injection causes a reduction of Notch activity and induction of Numb accumulation at 36 hpf. (A) Tg(Tp1:VenusPest) embryos were injected with lnx2a-MO (A2 and A4). Embryos were stained with anti-Pdx1 and anti-Numb antibodies and analyzed by confocal microscopy; Confocal images at 24 and 36 hpf are shown along three axes. (B) Total number of Notch-ON (Tp1: VenusPest+) cells and Numb positive cells were counted in all Z sections of 5-10 embryos for each category. N.S, not significant; ***, P < 0.001; Scale bar, 10 µm.
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