Fig. 10

Fig. 10

Mutational analysis of the 133-bp promoter. (A) Fertilized one-cell eggs were injected with the 133-bp promoter construct (WT) or its mutants with point mutations in either one or both of the GATA #1 and #2 elements. EGFP signals in the embryos were analyzed at 1, 2 and 3 dpf. The stacked bar charts represent the percentages of the following four groups of embryos: no EGFP expression (blue), cardiac-specific EGFP expression (green), EGFP expression in the heart and trunk muscle (red), and dead embryos (white). Data are expressed as the mean ± SEM of three independent experiments. (B–D) Representative images of the trunk region of 2-dpf embryos injected with the 133-bp promoter constructs having mutations in GATA #1 (B), GATA #2 (C), and both GATA #1 and GATA #2 (D). The asterisks indicate yolk sac autofluorescence. Bars, 0.2 mm.