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Fig. 4
Candidate genes are expressed in cell populations located adjacent to Hcrt neurons.
(A–I′′) Fluorescent ISH and immunofluorescence experiments in 2 dpf larvae and adult hcrt:EGFP zebrafish showing three candidate genes that are expressed adjacently to Hcrt neurons within the hypothalamus. (A–A′′, D–D′′, G–G′′) Dorsal view of the heads showing the whole expression pattern of the genes in 40 µM z-stack. (B–B′′, E–E′′, H–H) Dorsal view of single 0.5 µM plane in 2 dpf larvae. (C–C′′, F–F′′, I–I′′) Dorsal view of single 0.5 µM plane in adult brain section.
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