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Fig. 3
Inhibition Downstream of the M-Cell Does Not Cause Startle Habituation
(A) A 30-stimulus assay (5 pulses at 2 min ISI and 25 pulses at 5 s ISI) induced robust startle habituation in head-restrained larvae. Error bars indicate SEM.
(B) 40× magnification images of bilateral M-cell GCaMP6s labeling demonstrate unilateral (bottom, 42%) and bilateral (top, 58%) M-cell firing (n = 50 responses from five fish; scale bar, 10 µm).
(C) The frequency of M-cell somatic Ca2+ responses during short-term habituation is strongly reduced during habituation. Error bars indicate SEM.
(D) Representative images show baseline and peak fluorescence for non-habituating (stim 5) and habituating stimuli (stim 18 and 26; scale bar, 10 µm).
(E) Representative images at 10× (left) and 63× (right) show pre-ablation labeling of CoLo interneurons and their absence 24 hr post-ablation. Asterisk highlights a GFP-labeled muscle cell to indicate the same segments are imaged pre- and post-ablation (scale bar 10 µm).
(F) Both control and CoLo-ablated larvae rapidly habituate to repeated acoustic stimulation. Error bars indicate SEM.