ZFIN Image: van Boxtel et al., 2015, Fig. S1

Image

Figure Caption

Fig. S1

Fgf signaling inhibition and Nodal signaling

(A) Expression of fgf3 and fgf8a in 40% epiboly embryos in the margin shown as WISH and sections.

(B) ta and lft1 expression in 40% epiboly embryos injected with control MO or a combination of fgf3 and fgf8a MOs.

(C) ta expression in wild type and maternal-zygotic (MZ) tdgf1^-/- mutants. fgf8a mRNA (25 fg, 250 fg or 2.5 pg) was injected into wild type or MZtdgf1^-/- mutant embryos, which were assayed by WISH for ta expression at 40% epiboly. ta is induced equally well in both backgrounds with 2.5 pg fgf8a mRNA.

(D) lft1 and ta expression following inhibition of Nodal signaling with SB-505124 from the 16-cell or dome stage. When inhibited from dome stage, lft1 expression is severely reduced, whereas ta expression is unaffected.

Acknowledgments

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Reprinted from Developmental Cell, 35, van Boxtel, A.L., Chesebro, J.E., Heliot, C., Ramel, M.C., Stone, R.K., Hill, C.S., A Temporal Window for Signal Activation Dictates the Dimensions of a Nodal Signaling Domain, 175-185, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell