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Fig. S4 Partial recovery of AChE activity and grade 2 phenotype in zebrafish larvae exposed to 1 µM chlorpyrifos-oxon after the transfer to clean water. (A) Analysis of the recovery of AChE activity shows a clear dependence on the grade of severity/chlorpyrifos-oxon (CPO) concentration. Larvae were exposed to 0.1 and 1 µM CPO for 24h and the resulting grades 1 (0.1 µM) and 2 (1 µM) were analysed (total n=192, with 16 individuals for each experimental group, mean ± s.e.m.). (B) Normal phenotype of a vehicle exposed 8- 11 dpf zebrafish larva. (C) Phenotype of three grade 2 larvae immediately after 24 h exposure to 1 µM CPO, and 24-72 h after the transfer to clean water. A partial rescue of the phenotype is evident 3 days after the transfer to clean water.