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Fig. S14 Grade 3 larvae exhibit severe impairment in the brain architecture. Dorsal view of 3 heads of 8 days post-fertilization control and grade 3 zebrafish larvae, after the staining of the axonal tracts with an acetylated alpha-tubulin (green) monoclonal antibody by whole-mount immunofluorescence. The severe damage in the axonal tracts found in the brain of grade 3 larvae is consistent with the results of the semithin sections and transmission electron microscopy (TEM).
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