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Fig. 5
Cyclopamine treatment leads to a dramatic reduction in the number of olig2-positive cells specified in the hindbrain. Cyclopamine (50µm) was applied to wild type embryos from 32hpf then embryos analysed for olig2 expression at 52hpf. Cyclopamine-treated embryos (B) show a greatly reduced number of olig2-positive cells (arrow) compared with 0.5% ethanol treated (A) and untreated (C) control embryos. n=40, 20 embryos per group, data pooled from 2 independent experiments.
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