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Fig. 1 Zebrafish Maid expression in adult tissues.
(A) Western blot to detect Maid in total protein isolated from the indicated tissues of adult zebrafish. Ponseau-S staining shows total protein bands. (B) Top: Hematoxylin and eosin (HE) staining of the whole body and liver of a WT zebrafish embryo at 30 dpf, and the liver of a WT adult zebrafish at 6 months of age. Bottom: Immunohistochemical staining to detect Maid in the zebrafish tissues in the top panel. Black arrow indicates the liver, where the highest level of Maid expression was observed. (C) Top: HE staining of zebrafish kidney at 6 months of age. Bottom: Immunohistochemical staining to detect Maid in the zebrafish kidney in the top panel. (D) Localization of Maid in zebrafish liver. Immunofluorescent analysis of Maid protein localization in untreated juvenile zebrafish liver, adult zebrafish liver, and a DEN-induced foci. Red, anti-zebrafish Maid antibody; blue, DAPI (visualization of nuclei).