|
Fig. 1
mecp2 is enriched in the embryonic and adult brain in zebrafish. (A–D) RNA in situ hybridization with mecp2 probe in one-cell (A), 12hpf (B), 24hpf (C) and 48hpf (D) wild-type (WT) embryos. Note that mecp2 is broadly expressed in one-cell to 24hpf embryos and is enriched in the developing brain of 48hpf embryos (C,D). (E) Immunostaining of frozen sections of the adult zebrafish brain with anti-Mecp2 and anti-Huc antibody. Mecp2 protein was localized in the nuclei of neurons in which Huc protein was highly expressed. (F,G) Immunostaining (with embryos) and western blot (with protein extracts) in the developing zebrafish brain at 48hpf with anti-Mecp2 antibody. Mecp2 protein was reduced in mecp2 morphants compared with wild-type embryos. β-actin was used as a loading control. Lateral views (B–D) are shown with anterior to the left; a dorsal view is shown in F.