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Fig. 7
(A) Double antibody staining of metamorphic Tg(TDL358:GFP) fish with α-Tjp1aC and α-GFP antibodies. Note: not all iridophores are expressing GFP due to transgenic line variegation. Scale bar: 100 µm. (B) Loose iridophores migrating over the dark stripe in 8.3 mm metamorphic Tg(TDL358:GFP) fish express GFP, but not Tjp1a, although the epithelial staining is still visible. Scale bar: 30 µm. (C) α-Tjp1aC staining in skin of adult wild type fish. The protein is detected in the sheet of dense S-iridophores of the light stripe, but not in L-iridophores (black arrowheads), loose iridophores (white arrowheads), melanophores or xanthophores. Scale bar: 100 µm. (D) Double antibody staining with α-Tjp1aC and α-GFP of skin of adult chimera, obtained by transplanting sbr;Tg(H2A:GFP) blastomeres into wild type blastula. Either GFP or Tjp1a was detected in cells, never both. Some sbr cells express no GFP due to variegation of the transgene expression. Scale bar: 30 µm.