PUBLICATION

Tight junction protein 1a regulates pigment cell organisation during zebrafish colour patterning

Authors
Fadeev, A., Krauss, J., Frohnhöfer, H.G., Irion, U., Nüsslein-Volhard, C.
ID
ZDB-PUB-150429-6
Date
2015
Source
eLIFE   4: (Journal)
Registered Authors
Fadeev, Andrey, Frohnhöfer, Hans Georg, Irion, Uwe, Krauss, Jana, Nüsslein-Volhard, Christiane
Keywords
ZO-1, cell biology, chromatophores, colour pattern formation, developmental biology, iridophores, stem cells, tight junction protein, zebrafish
MeSH Terms
  • Animals
  • Body Patterning/physiology*
  • Cell Movement/physiology
  • Metamorphosis, Biological/physiology*
  • Pigmentation/physiology
  • Zebrafish
  • Zebrafish Proteins/metabolism*
  • Zonula Occludens-1 Protein/metabolism*
PubMed
25915619 Full text @ Elife
Abstract
Zebrafish display a prominent pattern of alternating dark and light stripes generated by the precise positioning of pigment cells in the skin. This arrangement is the result of coordinated cell movements, cell shape changes and the organisation of pigment cells during metamorphosis. Iridophores play a crucial part in this process by switching between the dense form of the light stripes and the loose form of the dark stripes. Adult schachbrett (sbr) mutants exhibit delayed changes in iridophore shape and organisation caused by truncations in Tight Junction Protein 1a (ZO-1a). In sbr mutants, the dark stripes are interrupted by dense iridophores invading as coherent sheets. Immuno-labelling and chimeric analyses indicate that Tjp1a is expressed in dense iridophores, but down-regulated in the loose form. Tjp-1a is a novel regulator of cell shape changes during colour pattern formation and the first cytoplasmic protein implicated in this process.
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Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
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Mapping