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Fig. 2
vsx2 gene expression in MO1a+MO1b embryos. In situ hybridization of vsx2 gene expression at 48 hpf (A and B). RT-qPCR was used to quantify RNA from three pools of 50 embryos collected from uninjected and morpholino injected embryos (C). There was a significant increase in vsx2 expression using Student′s t-test (p<0.05). Standard deviation was calculated from three replicate experiments.
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Reprinted from Developmental Biology, 402(2), Wong, L., Power, N., Miles, A., Tropepe, V., Mutual antagonism of the paired-type homeobox genes, vsx2 and dmbx1, regulates retinal progenitor cell cycle exit upstream of ccnd1 expression, 216-28, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.