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Fig. 1
(a) Ancora Genome browser window of the human SLIT3 gene on chromosome 5, harboring miR218-2 in intron 14. The red-yellow colored hill represents the density of noncoding sequences that are conserved with the mouse genome (minimum identity 96% over 50 bp; illustrated as yellow bars). The highest density is ~100 kb distant of miR218-2. Conservation with the zebrafish genome is shown below. Only two HCNEs (80% identity over 50 bp) are present (illustrated by the blue bars). (b,c) miR218 LNA and slit3 in situ hybridization, shown are 4 dpf zebrafish larvae. Labeled structures are indicated unilaterally with black abbreviations. Abbreviations in grey are used to describe anatomical features. Abbreviations: ce, cerebellum; f, fin; fp, floorplate; h, heart; hb, hindbrain; mb, midbrain; mp, midbrain basal plate; NIII, oculomotor nerve nucleus; NIV, trochlear nerve nucleus; NV, trigeminal nerve nucleus; NVII, facial nerve nucleus; NX, vagal nerve nucleus; ph, pharyngeal arches skeleton; tec, tectum; vn, vagal nucleus.