ZFIN Image: Dalgin et al., 2015, Fig. S7

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Figure Caption

Fig. S7

Label retaining cell analysis confirmed that early (dorsal) and late (ventral) born endocrine cells are similarly affected in Neurod morphants. Confocal images (merged z-stacks) of representative 60 hpf Tg(neurod:EGFP) embryos injected with H2B-RFP mRNA (red). GFP channel is not shown and hormone markers are false colored in green for presentation. Whole mount immunolabeling for insulin (green, , I-L), glucagon (green, M-P, U-X). Control (A, E, I, M, Q, U), Neurod UTR MO 2 ng (B, F, J, N, R, V), 4 ng (C, G, K, O, S, W) or 8 ng (D, H, L, P, T, X) injected specimens. Representative results of label retaining and non-label retaining cells are shown from two independent experiments. Arrowheads indicate label retaining dorsal bud-derived endocrine cells and arrows indicate non-label retaining ventral bud-derived endocrine cells. White scale bar=10 µm.

Acknowledgments

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Reprinted from Developmental Biology, 402(1), Dalgin, G., Prince, V.E., Differential levels of Neurod establish zebrafish endocrine pancreas cell fates, 81-97, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.