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Fig. S6
Neurod is also required for differentiation of endocrine cells from the ventral bud. Single channel confocal images (merged z-stacks) of Fig. 4 are shown. Whole mount immunolabeling for glucagon (red, E-F), insulin (red, M-P), somatostatin (red, U-X), GFP (green). Control (A, E, I, M, Q, U), Neurod UTR MO 2 ng (B, F, J, N, R, V), 4 ng (C, G, K, O, S, W) or 8 ng (D, H, L, P, T, X) injected specimens.
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Reprinted from Developmental Biology, 402(1), Dalgin, G., Prince, V.E., Differential levels of Neurod establish zebrafish endocrine pancreas cell fates, 81-97, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.