ZFIN Image: Dalgin et al., 2015, Fig. S10

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Figure Caption

Fig. S10

Increased Neurod levels do not promote beta to alpha cell fate change. (Top panel) Transplantation strategy; Tg(mnx1:GFP) donor cells were used to follow the fate of transplanted cells in endoderm deficient wild type hosts. Control or Neurod overexpressing donor cells were mosaic; Tg(mnx1:GFP) (green) or non-Tg(mnx1:GFP) (black). (A-I) Confocal images (merged z-stacks) of representative 52 hpf unmanipulated Tg(mnx1:GFP) specimens (A-C), chimeric specimens in which the entire endoderm is derived from control (D-F), or neurod mRNA injected (G-I) donor cell transplants. Embryos are immunolabeled for EGFP (green), for glucagon (red; A, D, G) and for insulin (blue; B, E, H). EGFP labels donor-derived beta cells (A, B, D, E, G, H). Merged images with all three colors are also shown (C, F, I). Results are representative of 4 chimeric specimens per group. White scale bar=10 µm.

Acknowledgments

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Reprinted from Developmental Biology, 402(1), Dalgin, G., Prince, V.E., Differential levels of Neurod establish zebrafish endocrine pancreas cell fates, 81-97, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.