Fig. 1
In situ hybridization and immunocytochemistry of anion exchanger 1b (AE1b) in zebrafish larvae.
AE1b mRNA was expressed by ionocytes (short arrows) and neuromasts (long arrows) of 72-h post-fertilization (hpf) larvae (A, B). No signal was found in larval skin with the sense probe (C). Confocal images of lateral-line neuromasts with the zebrafish AE1b antibody (D, E). AE1b was expressed on the apical portion of neuromasts in 96-hpf larvae (arrows, D). Magnification of the neuromast apical membrane showing that AE1b was expressed in stereocilia (arrows, E). The morphology of stereocilia was verified by an actin antibody (arrows, F).