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Fig. 2
SAC acquisition does not rely on zygotic transcription.
1-cell stage embryos were injected with α-amanitin as indicated, treated with or without nocodazole at 3.25 HPF for 45 min, fixed at 4 HPF, and stained for pH3 and DNA. Representative images are shown (A). The percent of nuclei positive for pH3 was calculated and averaged over multiple embryos (B, ne18 for each condition). Error bars indicate s.e.m, calculated over three independent experiments; scale bar 20 µm.
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