|
Fig. 3
Additional phenotypes of the zebrafish morphants of ttc4, -9c, -25, -36, and -39c.
(A) Typical otolith morphologies at 72 hpf. The otic vesicle normally contains two otoliths as shown. Otoliths that show differences in number, size and/or position were considered as abnormal. (B) Quantification results for abnormal otoliths in the indicated morphants. (C) and (D) Hydrocephalus formation (arrows in the bright-field images and asterisks in the histochemical sections) in the indicated morphants at 72 hpf. (E) and (F) The ttc25 and ttc4 morphants tended to develop pronephric cysts (arrows in the bright-field images and asterisks in the histochemical sections) at 72 hpf. (G) and (H) Left-right asymmetry patterning was disturbed in the indicated ttc morphants at 30 hpf. The cmlc2 probe was used to label the heart tube (arrows) in whole-mount in situ hybridization. All the quantification results were based on three independent experiments. Student’s t-test, ***P < 0.001. Error bars represent s.d.