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Fig. 8
Non-function of Wnt8a results from impaired interaction with its receptor in the target tissue.
Single confocal section showing a mosaic expression of Wnt8a-Venus (A–C) or Wnt8aCY-Venus (D–E) in embryos injected with Fzd9b-mRFP mRNA. Co-expression of Wnt8a-Venus and Fzd9b-mRFP induces the formation of membrane clusters which contain both proteins (arrowheads in A–C). Cluster formation is not observed after co-expression of Wnt8aCY-Venus with Fzd9b-mRFP (D-E). (A,D) Green channel, (B,E) red channel, (C,F) overlay.
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