ZFIN Image: Wang et al., 2014, Fig. 4

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Fig. 4

Time-lapse imaging of neutrophil migration in vivo. The ventral fin of 6-dpf larvae was injured by a sharp syringe needle, and chemotaxis of neutrophils were imaged by spinning disk microscope. (A) In a control larva treated with DMSO, neutrophil migration was not directed until 10 minutes after fin injury. Thereafter, they migrated actively towards the wound site (red asterisk; 25 and 30 minutes); by 40 minutes after injury, around eight neutrophils were recruited. (B) In contrast to the control, neutrophils treated with PF1052 remained stationary during 40 minutes of imaging; they appeared round and barely formed any pseudopods. (C) Higher magnification shows that DMSO-treated neutrophils actively formed multiple pseudopods and this was impaired in PF1052 treated-neutrophils (D).

Acknowledgments

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