Fig. 4
Estimation of transcript export delays of Nrarp in the mouse PSM. (A, i) Maximum z-projection of FISH against Mouse Nrarp. Segmentation of higher magnification FISH images was conducted as in Fig. 2B. An example source image (ii) and detailed view of the source image indicated by the hatched area (ii2) are shown, along with the segmented images representing unspliced nuclear pre-mRNA (iii), spliced nuclear mRNA (iv), and spliced cytoplasmic mRNA (v). Average z-projections are shown. (B) Intensity plots of images in A, iii–v measured from the posterior to anterior of the expression domain, averaged across the width of the PSM.