ZFIN Image: George et al., 2015, Fig. 8

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Fig. 8

Adult rescued nkx2.5^-/- fish exhibit normal cardiac morphology and chamber identity. Whole mount images of 7-month-old non-transgenic (A,B) and Tg(hsp70l:nkx2.5-EGFP) (C-F) wild-type (A-D) and nkx2.5^-/- (E,F) hearts. MF20/S46 immunofluorescence distinguishes ventricular (red) from atrial (green) myocardium. Dissected hearts are positioned ventrally with arterial poles to the top. “A” denotes atrium, “V” denotes ventricle, and “B” denotes bulbous arteriosus. All embryos were originally heat-shocked at 21 somites. All fish were genotyped prior to dissection for morphometric analyses. (A,C,E) Similar cardiac morphology is depicted in non-transgenic and transgenic wild-type and transgenic rescued adult nkx2.5^-/- hearts. (B,D,F) Cardiac chamber identity is maintained following heat shock at 21 somites in non-transgenic and transgenic wild-type and transgenic rescued adult nkx2.5^-/- hearts. (G) Bar graph indicates ventricle length (VL), ventricle width (VW) and bulbous arteriosus length (BAL) in non-transgenic wild-type (n=8), transgenic wild-type (n=7), and transgenic nkx2.5^/ (n=2) fish. Mean and standard error of each data set are shown with detection of statistically significant differences between VL and VW of transgenic rescued nkx2.5^-/- and non-transgenic wild-type fish (p<0.01).

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Acknowledgments

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Reprinted from Developmental Biology, 400(1), George, V., Colombo, S., Targoff, K.L., An early requirement for nkx2.5 Ensures first and Second heart field ventricular identity and cardiac function into adulthood, 10-22, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.