Fig. 4

Fig. 4

Vitamin K reduces hypermineralisation in gräte and dragonfish mutants. (A) Representative images of grt^-/- embryos after treatment with vitamin K or warfarin from 4-8dpf, showing that vitamin K reduces hypermineralisation, whereas warfarin exacerbates it. (B,C) Quantification of Alizarin Red staining in A reveals significant rescue of the phenotype by vitamin K (n = 20 embryos per group). (D) Dgf^-/- embryos treated with vitamin K or warfarin from 4-8dpf. (E,F) Quantification of Alizarin Red staining in D reveals a significant beneficial effect of vitamin K; results are similar to those seen in grt^-/- embryos (n = 24 per group). (G) Alizarin Red staining of dgf^-/- embryos exhibiting ectopic mineralisation in the ventral skin. Administration of vitamin K did not affect the incidence (H) or the extent (I) of this mineralisation.