Fig. 5
Normal macular development is required for normal expression of otog and tecta. (A) otog mRNA expression at the poles of the OV of a 21hpf wild-type (LWT strain) OV. (B) Expression of otog was reduced in the OV of atoh1b morphants. (C) Expression of tecta at the poles of a 21hpf wild-type OV. (D) tecta expression was not detected in the OV of atoh1b morphants. (E) otog expression in the utricular macula of a 31hpf phenotypically wild-type sibling embryo. (F) otog expression was reduced in the utricular macula of a 31hpf mib mutant embryo. (G) tecta expression in the utricular macula of a 31hpf phenotypically wild-type sibling embryo. (H) tecta expression was reduced in the utricular macula of a 31hpf mib mutant embryo. Weak expression remained in the saccular macula (out of focus). Dorsal (A-D) and lateral (E-H) views, with anterior to left. Scale bar: 50µm for A-H.