Fig. 2

Fig. 2 Anillin is required for the apical distribution of F-actin and Par3. (A) Frames from supplementary material Movie 3. F-actin accumulates at the Anillin-eGFP-labelled midbody (arrow) at the end of cytokinesis. (B) Asymmetric (CtrlMO; frames from supplementary material Movie 4) and symmetric (anlnMO; frame from supplementary material Movie 5) distribution of apical F-actin accumulation. (C) Position of the apical F-actin-rich domain (CtrlMO, n=14; anlnMO, n=13) (as described in Fig. 1E-F and supplementary material Fig. S3); the frequency of asymmetry is 57% in CtrlMO and 15% in anlnMO (Wilcoxon Mann–Whitney test, *P<0.05). (D) Symmetric versus asymmetric inheritance of Par3 in CtrlMO (frame from supplementary material Movie 6) and anlnMO (frame from supplementary material Movie 7) injected embryos (two-tailed Fisher′s exact test, ****P=10⁴, CtrlMO, n=15; anlnMO, n=15). All images represent a single z-plane of a confocal stack. The apical surface of the retinal neuroepithelium is to the top. Scale bars: 4.8µm in A; 5µm in D.