Fig. 6

Fig. 6 Effect of VEGF-A supply on WA-25-induced neovascularization blockade in vivo (A) Effect of exogenous VEGF-A on WA-25-induced angiogenesis blockade on the microvessel sprouting in aorta rings. Rat aortic rings were placed in Matrigel and treated with VEGF-A or WA-25. The effect of WA-25 on the formation of vessel sprouts from various aortic samples was observed on Day 7; (B) Effect of exogenous VEGF-A on WA-25-induced angiogenesis in Tg(kdrl:mCherry^ci5-fli1a:negfp)^y7 zebrafish embryos. Embryos were treated with WA-25 (50 µM) at 6 hpf with or without VEGF (10 ng/mL) and then imaged at 24 hpf. The number of endothelial cells on the ISV was determined by counting the green nuclei over the red blood vessel. Data are represented as mean ± SD (n = 12). Asterisks indicate statistical significance versus control (* p < 0.05 and ** p < 0.01).