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Fig. 5 Effects of exogenous VEGF on WA-25-induced neovascularization blockade in vitro. After treatment with WA-25 (20 µM) with or without VEGF (10 ng/mL), the effects of WA-25 on VEGF-induced proliferation (A); migration (B); and tube formation (C) were determined in HUVECs; (D) Immunoblot analysis of VEGF and VEGFR2 expression in HUVECs after treatment with WA-25 (20 µM) with or without VEGF (10 ng/mL) for 24 h. Data are represented as mean ± SD of quadruplicate experiments. Asterisks indicate statistical significance versus control (* p < 0.05 and ** p < 0.01). NS, not significant.
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