Fig. 2
Effects of DDT and lindane on the hatching and abcc4 expression of zebrafish embryos.
(A and B) Hatching rates of 96-hpf embryos exposed to DDT or lindane at indicated concentrations. (C and D) Total RNAs of zebrafish embryos in (A) and (B) were extracted for real-time PCR. (E) Embryos treated with 0.05 µg/L DDT or 0.01 µg/L lindane from 24 to 96 hpf were subjected to WISH analysis of induced abcc4 expression, respectively. CK represents the untreated control. Black arrows point to intestine. All values are expressed as mean ± standard deviation, n = 3. Significant differences are indicated by *p<0.05 and **p<0.01.