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Fig. 6 Overexpressing spi1b mRNA could rescue the depleted expression of mfap4 or mpx in ncor2 morphants at 24 hpf. A–F: Whole-mount in situ hybridization was performed to examine the expression of mfap4 (A–C) and mpx (D–F). Embryos were microinjected with control MO (A,D), ncor2 MO (B,E) and ncor2 MO plus spi1b mRNA (C,F), respectively. G: The scatter plot showing the number of mfap4+ cells in the embryos microinjected with control MO, ncor2 MO, and spi1b mRNA plus ncor2 MO, respectively. H: The scatter plot showing the number of mpx+ cells in the embryos microinjected with control MO, ncor2 MO, and spi1b mRNA plus ncor2 MO, respectively.