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Fig. 1 Co-localization gnrh3 mRNA and GFP in transgenic fish expressing GFP-LacZ under the control of the gnrh3 promoter.
A–D, 3 dpf, E–H, 4 dpf. A and E, bright field (BF) view of the embryos. B and F, green color indicated gnrh3 mRNA detection by in situ hybridization. C and G, GFP immunostaining is shown as red signal. D and H, the merged pictures show co-localization of gnrh3 and GFP signals in the olfactory bulb (OB) at 3 dpf and 4 dpf. The anterior is to the left in all panels, and arrows point to the olfactory bulb.
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