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Fig. 3 Ribeye B-domain/CtBP2s localizes to synaptic ribbons at 3 dpf.
Representative images of immunolabel or fluorescent tag in posterior lateral line NM1 hair cells of 3 dpf larvae. Scale bars: 3 μm (main panels), 1 μm (insets). (A) Ribeye (B-domain)-GFP (cyan) and Ribeye a antibody labeling of synaptic ribbons (magenta). Note that B-domain-GFP does not completely colocalize with Ribeye a immunolabel. (B) Ribeye (B-domain)-myc (cyan), Ribeye a (blue), and Ribeye b (red) immunolabel. Dashed circles indicate nuclear localization of B-domain. Note that Ribeye a and b immunolabel colocalizes with each other (magenta), but only partially colocalizes with B-domain (white). Red arrows indicate resolvable indentations in the synaptic ribbons that do not contain exogenous B-Domain, but do contain Ribeye. (C) Ribeye a (full length)-mcherry (cyan) and Ribeye b immunolabel (magenta). Full length exogenous Ribeye a-mcherry appears throughout the synaptic ribbon and colocalizes with Ribeye b immunolabel (white).