Fig. 3
Parapineal cell numbers are unaffected in the absence of epithalamic Nodal signalling. (A,A2) Frontal view of the epithalamus showing in situ hybridisation against pitx2c at 24 hpf in mock-treated embryos (A) or those treated with 100 μM SB431542 (A2). (B,B2) Single confocal sections of the parapineal showing the expression of GFP from the Et(krt4:EGFP)sqet11 transgene in mock-treated embryos (B) or those treated with 100 μM SB431542 (B2). The outline of the pineal gland is highlighted with a dotted line. (C) Counts of cell expressing the Et(krt4:EGFP)sqet11 transgene in mock-treated and SB431542-treated embryos. The number of GFP-positive cells is unaffected by treatment with SB431542. Error bars represent s.d.