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(A–F) Whole mount ISH for bmpr1ab (A, B), bmpr1ba (C, D), and bmpr1bb (E, F) expression, lateral views, anterior to the left. (G) Histogram quantifying the number of dntcf3+ embryos with reduced expression. (H,I) qPCR analysis of Bmp receptor expression levels in dntcf3+ embryos at different times post heat shock, normalized to nontransgenic, heat-shocked controls, with ef1alpha as an internal control. * P<0.05, ** P<0.001. Abbreviations: e, eye; nc, neural crest. Scale bar: 100 µm.
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